Tag

Part:BBa_K3946003:Design

Designed by: Fiona Bunn   Group: iGEM21_Edinburgh   (2021-10-19)


L2NC+linker (C)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Addition of recognition sites of Bsa1 and BsmB1 enzymes with overhangs. And have a start codon but not a stop codon because would be linked to the terminator gene as a C-terminal tag.

Source

Escherichia coli

References

https://doi.org/10.1021/acsami.0c03804